结直肠癌肝转移动物模型的论文.docVIP

　　结直肠癌肝转移动物模型的论文 【摘要】 目的 比较几种结直肠癌肝转移动物模型的建立方法，确定一种比较适合的结直肠癌肝转移动物模型。方法 以balb/c鼠为研究对象，随机分组，分别经脾脏、直肠、腹腔按不同剂量(0.1 ml、0.2 ml、0.3 ml、1.0 ml) (浓度为1×106个/ml)注入小鼠结肠腺癌细胞(ct26)悬液，其中腹腔组无1.0 ml剂量，卡方检验比较三组动物模型组内及组间肝转移率。结果 三种方法均能复制出结直肠癌肝转移动物模型，脾脏组0.1 ml、0.2 ml、0.3 ml、1.0 ml肝转移率分别为50.0%、77.2%、50.0%、27.0%;直肠注射组0.1 ml、0.2 ml、0.3 ml、1.0 ml肝转移率为50.0%、53.1%、16.7%、6.7%;腹腔注射组0.1 ml、0.2 ml、0.3 ml肝转移率为10.0%、22.2%、10.0%。结论 经脾脏注射0.2 ml(1×106个/ml)balb/c鼠结肠腺癌细胞株(ct26)是一种建立结直肠癌肝转移动物模型成功率较高的方法。而经肛门直肠注射0.1 ml或0.2 ml(1×106个/ml)balb/c鼠结肠腺癌细胞株(ct26)是一种建立结直肠癌肝转移动物模型简便及符合结直肠癌肝转移规律的方法。 【关键词】 balb/c鼠;balb/c鼠结肠腺癌细胞株(ct26);结直肠癌;肝转移;动物模型 establishment of animal models in colorectal cancer etastasis 　　yang jianfeng,zhang sen,gao feng,et al.department of the colorectal and anal surgery, the first affiliated hospital of guangxi medical university, naning 530021,china 　　[abstract] objective to define a suitable method of establishing animal model of colorectal cancer etastasis by paring several methods. methods balb/c mouses ly into 11 groups by doses of adenocarcinoma cells and methods of injection, that is 0.1 ml, 0.2 ml, 0.3 ml, 1.0 ml(colon adenocarcinoma cells in 1×106/ml) by spleen, rectum, peritoneal cavity(no 1.0 ml dose group in peritoneal group). the rate of liver metastases ethods al model of colorectal cancer etastases. the liver metastasis rates l, 0.2 ml, 0.3 ml, 1.0 ml doses by spleen; there l, 0.2 ml, 0.3 ml, 1.0 ml doses by rectum, and 10.0%, 22.2%, 10.0% in 0.1 ml, 0.2 ml , 0.3 ml doses through peritoneal cavity. conclusion inject through spleen l(1×106/ml) is a effective al model of colorectal cancer etastasis. inject through rectum l or 0.2 ml(1×106/ml) ct26 is a simple al model of colorectal cancer etastasis , and folloetastases of colorectal cancer also. 　　[key ouse; balb/c mice colon adenocarcinoma cell line (ct26);colorectal cancer; liver metastasis; animal model 　　结直肠癌是消化道最常见的恶性肿瘤之一, 2008年《中国卫生统计提要》示结直肠癌病死率位居恶性肿瘤第5位[1]。.目前国内以每年4.2%的速度上升[2]，全世界结直肠癌的发病率以3%的速度上升[3]。影响结直肠癌患者预后的最大问题就是肿瘤复发或