- 1、本文档共28页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
Post-transcriptional gene silencing;;Posttranscriptional gene silencing;Other names of post-transcriptional gene silencing (PTGS) :
– gene silencing
– RNA silencing
– RNA interference
– In certain fungi: quelling
RNAi can spread throughout certain organisms
(C. elegans, plants).
;Short history of post-transcriptional gene silencing
Definition: the ability of exogenous double-stranded RNA (dsRNA) to suppress the expression of the gene which corresponds to the dsRNA sequence.
1990 Jorgensen :
Introduction of transgenes homologous to endogenous genes often resulted in plants with both genes suppressed!
Called Co-suppression
Resulted in degradation of the endogenous and the
transgene mRNA
;1995 Guo and Kemphues:
injection of either antisense or sense RNAs in the germline of C. elegans was equally effective at silencing homologous target genes
1998 Mello and Fire:
-extension of above experiments, combination of sense and antisense RNA (= dsRNA) was 10 times more effective than single strand RNA;What is RNA interference /PTGS?
dsRNA needs to be directed against an exon, not an
intron in order to be effective
homology of the dsRNA and the target gene/mRNA is
required
targeted mRNA is lost (degraded) after RNAi
the effect is non-stoichiometric; small amounts of
dsRNA can wipe out an excess of mRNA (pointing to
an enzymatic mechanism)
ssRNA does not work as well as dsRNA
; double-stranded RNAs are produced by:
– transcription of inverted repeats
– viral replication
– transcription of RNA by RNA-dependent RNA-
polymerases (RdRP)
double-stranded RNA triggers cleavage of
homologous mRNA
PTGS-defective plants are more sensitive to infection
by RNA viruses
in RNAi defective nematodes, transposons are much
more active;;;;;Dicer
Double-stranded RNA triggers processed into siRNAs
by enzyme RNAseIII family, specifically the Dicer family
Processive enzyme - no larger intermediates.
Dicer family p
您可能关注的文档
- MethodologyofSodium.PDF
- MicrowaveAssistedPreparationand.PDF
- MINISTERULFAMILIEI,TINERETULUIISPORTULUIAL.ppt
- MK04蛋白分子量Marker(12条带).pdf-上海歌凡生物科技有限.PDF
- Mn2+金属离子配位机理.PDF
- MSALRadiationOncologyResearchPartnership.ppt
- MS测定聚丙烯输液瓶中镁、铝元素的含量及提取迁移测定.PDF
- MT7968AL.PDF
- Mutationscreeningofthesodium.PDF
- mutualinconsistencyindistributedsystems(Parker,Popek,.ppt
1亿VIP精品文档
相关文档
最近下载
- 超长航时太阳能无人机关键技术综述.docx VIP
- 南京林业大学《概率论与数理统计》2022-2023学年第一学期期末考试试卷.docx
- 2024年浙江省中等职业学校职业能力大赛(学生职业素养类)“创新创效创业”项目(学校选拔)试题库(带答案).docx
- Unit4中译英单词默写表Excel版 译林牛津版高中英语选择性必修第一册.docx VIP
- 吉林大学2022-2023学年第2学期《高等数学(下)》期末试卷(A卷)附标准答案.pdf
- 宁煤公司考试试卷题库答案.pdf
- SSD 548U故障解列装置说明书_V1.00【常规标准版】【一元件】【低频低压】【过频过压】【零序过压】.pdf
- 基于stm32的数码相框设计与实现-外文翻译.doc
- PCB出货检验报告_2.doc
- 分析化学(仪器分析)教学课件(全).ppt
文档评论(0)