响应面法优化复序橐吾中多酚提取工艺.PDF

第8 卷 第17 期 Vo l.8 No .1 7 2015 年 9 月 September 2015 响应面法优化复序橐吾中多酚提取工艺 赵 岩 1，彭 蕾 1，郭世杰 2，蔡恩博 1，刘双利 1，杨 鹤 1，张连学 1* （1. 吉林农业大学中药材学院，长春 130118； 2. 吉林大学第一医院新生儿科，长春 130021） 摘要：目的：研究复序橐吾多酚的最佳提取工艺。方法：以复序橐吾为材料，应用岭回归和响应面分析法，研 究乙醇浓度、超声时间、液固比及其交互作用对多酚提取率的影响。结果：复序橐吾中多酚的最佳提取条件 为乙醇浓度 41.6%（体积比）、液固比 228.3 mL/g、提取时间 26.7 min. 在此条件下，复序橐吾中多酚提取率达 4.48%（质量比）. 结论：研究建立的工艺模型适用于复序橐吾多酚的提取，可用于实际生产。 关键词：中药化学；复序橐吾；多酚；提取工艺；响应面分析；岭回归 中图分类号：R284.3 文献标识码：A 文章编号：1674-2850(2015)17-1787-08 Optimization of extraction process for polyphenols from Ligularia jaluensis by response surface methodology ZHAO Yan1, PENG Lei1, GUO Shijie2, CAI Enbo1, LIU Shuangli1,YANG He1, ZHANG Lianxue1 (1. Chinese Medicine Material College , Jilin Agricultural University, Changchun 130118, China; 2. Department of Neonatology, The First Hospital of Jilin University, Changchun 130021, China) Abstract: Objective: To study the best extraction technique conditions of polyphenols from Ligularia jaluensis. Methods: Using the combination of ridge regression and response surface analysis method, taking L. jaluensis as materials, the interaction effects of the ethanol concentration, ultrasonic time and liquid-solid ratio on total polyphenols extraction yield were studied. Results: The results show that the optimal levels of liquid-to-material ratio, extaction time and ethanol concentration were determined to be 228.3 mL/g, 26.7 min and 41.6% (volume ratio). Under these conditions, the extraction yield of total polyphenols is 4.48% (mass ratio). Conclusion: The research process model is suitable for polyphenols extraction from L. jaluensis, which can be used in practical production. Key words: pharmaceutical chemistry