益气化瘀补肾方治疗气虚血瘀肾虚型颈椎病大鼠的机制研究的论文.docVIP

　　益气化瘀补肾方治疗气虚血瘀肾虚型颈椎病大鼠的机制研究的论文 【摘要】 目的：研究益气化瘀补肾方治疗气虚血瘀肾虚型颈椎病大鼠的作用机制。 方法：选择3月龄雌性spf级sd大鼠30只，随机分为正常组、模型组和益气化瘀补肾方组，每组10只。气虚血瘀肾虚型颈椎病模型通过病、证模型复合而成。益气化瘀补肾方组采用益气化瘀补肾方治疗1个月。通过放射免疫学、组织病理学、免疫组织化学和分子生物学等技术研究益气化瘀补肾方的作用机制。结果：与正常组比较，模型组动物出现精神萎靡、少动，舌质、尾色瘀紫，体质量减轻，子宫及附件质量降低，环磷酸腺苷与环磷酸鸟苷及其比值无明显差异，血黏度、血小板表面α颗粒膜糖蛋白（alphagranular membrane protein, cd62p）增高，血清雌二醇（estradiol, e2）降低。益气化瘀补肾方组与模型组比较，除子宫及附件质量外，上述指标均有不同程度改善。he染色显示模型组颈椎间盘高度降低，纤维环出现裂隙，胶原纤维排列紊乱，髓核减少，软骨终板变薄；益气化瘀补肾方组椎间盘高度正常，纤维环排列稍紊乱，髓核结构良好，软骨终板变化不明显。与正常组比较，模型组ⅱ型胶原表达减少；益气化瘀补肾方组ⅱ型胶原在纤维环的表达比模型组有所增加。模型组与正常组比较ⅱ型前胶原基因和基质金属蛋白酶抑制剂1(tissue inhibitor of metalloproteinase1, timp1))基因表达降低（plt;0.01），益气化瘀补肾方组与模型组比较，ⅱ型前胶原基因和timp1基因表达增高，基质金属蛋白酶13基因表达降低（plt;0.05, plt;0.01）。. 结论：益气化瘀补肾方通过调节免疫、凝血和内分泌系统等改善模型动物气虚血瘀肾虚状态，通过调节椎间盘细胞外基质以及相关金属蛋白酶延缓椎间盘退变。 【关键词】 益气化瘀补肾方; 气虚; 血瘀; 肾虚; 颈椎病; 大鼠 ed. 2009; 7(1): 5358. received may 30, 2008; accepted july 24, 2008; published online january 15, 2009. indexed/abstracted in and full text linkout at pubmed. journal title in pubmed: zhong xi yi jie he xue bao. free full text (html and pdf) is available at . fopen access mechanism of yiqi huayu bushen recipe in treating cervical syndrome in rats edicine, shanghai 200032, china objective: to explore the mechanism of yiqi huayu bushen recipe (yhbr), a pound traditional chinese herbal medicine, in treating cervical syndrome (cs) ethods: a total of 30 threemonthold female spraguedaly divided into normal control group, untreated group and yhbr group; there odel of cervical syndrome e models. after onemonth yhbr treatment in yhbr group, all rats , plasma and cervical intervertebral discs munology, histopathology, immunohistochemistry, etc. results: pared al control group, rats in the untreated group shoovement, bluishpurple tongue and tail and onophosphate/cyclic guanosine monophosphate orheological parameters and the expression of alphagranular membrane protein (cd62p) estradiol prove the above indexes except for the inish of pulposus nucleus,