131I-labeled anti-CD20 monoclonal antibody in tumor-bearing nude mice Radioimmunoimaging.docVIP

 PAGE \* MERGEFORMAT 17 131I-labeled anti-CD20 monoclonal antibody in tumor-bearing nude mice Radioimmunoimaging Authors: Zuo-Qiang, Zhang 1, Mr Chan Kam Zhang, Rong-Cheng Luo 【Abstract】 Objective To investigate the 131I  anti  CD20McAb through after intratumoral injection of cell lines bearing human Burkitt’s lymphoma Raji cells in nude mice xenograft Radioimmunoimaging. Methods 131I marker signs are IODO  GEN iodide marker; injection marker 1,3,7,15 days after the tumor-bearing nude mice after SPECT imaging of living killing, scaler measure and calculate tumor, blood, etc. 12 kinds of organ or tissue of the% ID / g values, according to MIRD formula recommended by the Committee tumors accumulated absorbed dose. The results of 131I  anti  CD20McAb intratumoral injection group, the SPECT imaging was superior to intraperitoneal injection of 131I  IgG group and the intratumoral injection group, the group tumor% ID / g values in the administration of 1,3 and 7 days after the first, respectively the latter two groups of 1.4 ~ 17-fold and 1.7 ~ 3.7 times the tumor involving the absorbed dose after administration of 3,7 and 15 days respectively, the latter two groups of 1.5 ~ 2.5 times and 6.0 ~ 12.6 times. Conclusion 131I  anti  CD20McAb within the means of delivery by the tumor can make the highest tumor uptake of radioactive substances, the use of the channels for the next step radioimmunotherapy provide an experimental basis. Keywords: 131I; anti-CD20 monoclonal antibody; non-Hodgkin’s lymphoma; radioimmunoimaging 1 Materials and methods 1.1 Material of anti-CD20 monoclonal antibody from Roche, mouse IgG for the SIGMA company’s products, Na131I solution purchased from Chengdu in the nuclear high-pass Isotopes Corporation, human Burkitt’s lymphoma cell line Raji cells from Shanghai Cell Institute. 1.2 monoclonal antibody and the activity assay of markers of anti-CD20 monoclonal antibody and mouse IgG and 131I tags