131I1H12 Preparation and A549 non-small cell lung cancer cells and the biological effect of the combination of.docVIP

 PAGE \* MERGEFORMAT 16 131I1H12 Preparation and A549 non-small cell lung cancer cells and the biological effect of the combination of Of: Yin Ning, Du Minghua, Zhong Ying, Qu ships [Abstract] Objective: To study the radionuclide Iodine (131I) labeled epidermal growth factor receptor monoclonal antibody (McAb) 1H12 experimental conditions to observe the product of 131I 1H12 labeled with A549 human lung cancer cell immune binding and biological effects. Methods: 131I labeled by Iodogen method 1H12, by in vitro binding assay of labeled antibody detection of the immune activity; by flow cytometry of different doses of 131I 1H12 (148,74,37 kBq), free 131I (148 kBq) and 1H12 (80 nmol ml-1) on A549 cells. Results: 131I 1H12 mark was 50.14%, specific activity 4.63 MBq g-1, radioactive concentration of 77.31 MBq ml-1, radiochemical purity was 96.92% .131 I 1H12 combined with the A549 cells was 61.12%; 131I 1H12 A549 induced apoptosis and cell cycle arrest in a dose-response relationship, the role of the 148 kBq131I 1H12 greatest effect, apoptosis rate was (44.35 + -3.31)%, G2 / M phase cell block rate (51.17 + -2.98)%. CONCLUSION: 131I 1H12 immune cells can be combined with the A549, and the regulation of cell cycle and induce apoptosis. [Keywords:] non-small cell lung cancer; epidermal growth factor; radioimmunotherapy; 131I; monoclonal antibody [Abstract] Objective: To investigate the experimental conditions of radionuclide iodine (131I) labeling epidermal growth factor receptor (EGFR) monoclonal antibody (McAb) 1H12, observe the cell binding function and biological effects of 131I 1H12 to human lung cancer cells A549. Methods : 1H12 was labeled with 131I by Iodogen method. The immunocompetence of 131I 1H12 was analyzed by cell binding test. The cell apoptosis and cell cycles of A549 were analyzed by flow cytometry assay with various doses of 131I 1H12 (148, 74, 37 kBq) , free131I (148 kBq), 1H12 (80 nmol ml-1) and equivalent medium. Results: The labe