5 The first integron-positive analysis of resistance mechanisms of Pseudomonas aeruginosa.docVIP

 PAGE \* MERGEFORMAT 16 5 The first integron-positive analysis of resistance mechanisms of Pseudomonas aeruginosa Author: Xiao Zeng Huang Ju-zhen Fu Qiang Shi Lei [Abstract] Objective 5 separated from the first category of integration of sub-clinical positive molecular biological characteristics of Pseudomonas aeruginosa. Methods PCR method to determine five of Pseudomonas aeruginosa integron containing the first category, and their integrated resistance gene cassette was amplified and sequenced by PFGE analysis of the five strains of homology. The results are the first five categories of Pseudomonas aeruginosa integron-positive strains and both carry 2360bp resistance gene cassette, including the termination of aacA4 and products containing mutations cmlA1. PFGE results showed that five of Pseudomonas aeruginosa from the same clone. Conclusion integrons in clinical multi-drug resistance of bacteria plays an important role, this study suggests that should strengthen the monitoring of bacterial resistance and to prevent the spread of hospital-related pathogens. [Keywords:] integration of the child; Pseudomonas aeruginosa; PFGE Currently, the mechanism of bacterial resistance has become a research hotspot. Carrying drug-resistant bacteria showed resistance gene is a major reason. Resistance gene, mainly through plasmids, transposons, and integrons etc. horizontal transmission, which combines the sub-horizontal spread is mediated by gram-negative bacteria, especially enteric bacteria and Pseudomonas bacteria are an important reason for multi-drug resistant . Has been confirmed that there are at least nine kinds of integration of the child, but play a leading role in consolidation for the first child [1,2]. The first integron mainly by two conservative side as well as between the conservative end of the resistance gene cassette composed of. 5 ‘ conservative end of a code of intI1 integrase gene and promoter, most of the 3’  conservative side there are three