5FU on SW480 cells Musashi1 gene expression in.docVIP

 PAGE \* MERGEFORMAT 5 5FU on SW480 cells Musashi1 gene expression in [Abstract] Objective: To observe the 5  fluorouracil (5  FU) on human colorectal cancer cell line SW480 role and Musashi  1 gene expression relationships. Methods: MTT assay at different time 5  FU on SW480 cell proliferation, flow cytometry side population (SP) cells and CD34 + CD44 + cell ratio, RT  PCR semi-quantitative detection of Musashi  1 gene expression. Results: 5  FU acting on SW480 cells, 24,48,72 h followed by half of the inhibition rate 32.12,16.2 and 6.8 mg * L-1, SP cell ratio, CD34 + CD44 + cells and Musashi  1 mRNA expression in the role of 5  FU after had increased. Conclusions: The normal intestinal stem cell marker Musashi  1 in colorectal cancer may become a stem cell-like tumor cell-specific markers, CD34, CD44 may have a similar potential. [Keywords:] colorectal cancer cell line SW480; Musashi  1 gene; cancer stem cells; 5  FU Colorectal cancer is a common one, its incidence and mortality rates have gradually rising trend. With regard to the pathogenesis of a number of hypotheses exist, one of which is ‘cancer stem cell hypothesis’. The hypothesis that there is a small group of tumors with a stem cell function in tumor cells, called cancer stem cells (cancer stem cells, CSCs), the existence of these CSCs may be the root causes of tumor, but also may be an important cancer drug resistance causes and origins of recurrence or metastasis. Studies have confirmed the existence of the normal intestinal crypt stem cells, but also confirmed that Musashi  1 is its specific marker. In this study, commonly used in colorectal cancer chemotherapy drug 5  fluorouracil (5  FU) effect on colorectal cancer cell line SW480, the main observed changes in expression of Musashi  1. 1 Materials and methods 1.1 material in human colorectal cancer cell line SW480 (Chinese Academy of Sciences Shanghai Institutes for Biological Sciences Cell Resource Center),