About Eag1 potassium channel expression in cervical cancer and the regulation of hypoxia.docVIP

 PAGE \* MERGEFORMAT 19 About Eag1 potassium channel expression in cervical cancer and the regulation of hypoxia Of: Lin Shuang, Li Li, Liu Yuan, Li Shulin [Abstract] Objective To study the different tissues Eag1 cervical lesions and the expression of hypoxia in cervical cancer Hela cell membrane Eag1 potassium channel expression and activity. Methods Immunohistochemical staining of different cervical tissue and cervical cancer Hela cells Eag1 protein expression; RT-PCR assay using different concentrations of hypoxia and hypoxia on Hela cells Eag1 mRNA expression. using whole cell patch clamp method to study hypoxia on Eag1 potassium ion channel activity. Results cervical intraepithelial neoplasia tissue Eag1 protein expression was significantly higher than the normal group, cervical carcinoma was significantly higher than Eag1 cervical intraepithelial neoplasia group; Eag1 expression increased gradually with increasing clinical stage, Eag1 expression pathological grade was no significant correlation. normal cultured cervical cancer Hela cells that express a Eag1; the deepening of its expression with hypoxia and hypoxia significantly increased time. Hela cells Eag1 potassium channels is voltage dependent, and yet live with delayed rectifier characteristics; mild hypoxia can Hela Eag1 potassium current strength increases, the activation time shortened. Conclusion Eag1 potassium ion channel protein may become the new biology of early cervical cancer screening and to determine Behavior Scale. [Keywords:] cervical cancer; potassium ion channel; Eag1 [Abstract] Objective Immunohistochemistry was employed to detect the expression of Eag1 protein in different cervix uteri tissues and CACX Hela cells.Methods We determined the effect of different hypoxia time and hypoxic concentration on the expression of Eag1 mRNA in Hela cell using RT-PCR. Meanwhile, whole cellular patch clamp recording was employed to investigate the effect of hypoxia on activity of Eag1