About DLXNP on focal cerebral ischemia reperfusion of tumor necrosis factor α expression and cell apoptosis.docVIP

 PAGE \* MERGEFORMAT 12 About DLXNP on focal cerebral ischemia reperfusion of tumor necrosis factor α expression and cell apoptosis OF: He Qian, Zhou Xiaoqing, Li Hua, Liu Wanghua, Liu Jianxin [Abstract] Objective DLXNP on cerebral ischemia, reperfusion neuronal apoptosis and tumor necrosis factor-@ (TNF-@) expression, and to explore its mechanism. Methods patching method Focal cerebral ischemia model, using TUNEL and immunohistochemistry were used to detect the sham group, model group, DLXNP group and the nimodipine group and the TNF apoptotic cells - @-positive cells. Results Compared with the sham operation group, model group, the number of apoptotic cells and TNF-@ expression was significantly increased (P lt;0.05, P lt;0.01), compared with model group, group DLXNP number of apoptotic cells and TNF-@ was significantly lower (P lt;0.05). Conclusions TNF-@ expression may be a reduction of DLXNP cerebral ischemia reperfusion injury in the molecular mechanism of apoptosis. [Keywords:] cerebral ischemia, DLXNP, apoptosis, tumor necrosis factor-@, rat Abstract: Objective To study the effects of Danlong Xingnao Tablet on neuronal apoptosis and expression of TNF-@ after focal cerebral ischemia reperfusion in rat hippocampus. Methods The model of middle cerebral arteries occlusion (MCAO) were performed with intraluminal filament blockade. By using in situ terminal deoxynucleotidyl transferase-mediated dUTP-biotor nick end labeling (TUNEL) and immunohistochemistry staining, the number of apoptotic cells and TNF-@ positive cells were detected in the sham-operated group, the ischemia reperfusion group, Danlong Xingnao Tablet group and nimodipine group after the middle cerebral arteries of SD rats occluded for 1 h and reperfused for 24 h. Results Compared with the sham operation group, the number of apoptotic cells and TNF-@ positive cells of the model group increased significantly (P lt;0.05, P lt;0.01). Compared with the model group, DLXNT intragastric ad