About PUVA on human leukemia cell apoptosis and Fas expression.docVIP

 PAGE \* MERGEFORMAT 11 About PUVA on human leukemia cell apoptosis and Fas expression Of: Chen Nannan, Huang Shilin, Sunningdale, Zhangde Jie, ZHANG Chen, Zhang Li [Abstract] Objective Photochemotherapy (PUVA) induced by human leukemia cells HL-60, K562, NB4 apoptosis when Fas expression. Methods in human leukemia cells with different concentrations of psoralen (PSO), to accept or not accept long-wave ultraviolet (UVA) irradiation of cultured cells were observed under the electron microscope ultrastructural changes of cells were detected by fluorescence quantitative PCR, the expression of Fas gene flow cytometry (FCM) detection of apoptosis and Fas protein expression, the use of multi- ANOVA was used for statistical analysis. Results PUVA treatment of human leukemia cell ultrastructure obvious morphological changes of apoptosis, PSO, UVA irradiation and PUVA increased the apoptosis rate could be increased Fas gene leukemia cells , protein expression, PUVA was significantly stronger than the role of the former two (P lt;0.01). Conclusion PUVA can induce apoptosis in human leukemia cells, the role of PSO and stronger than UVA radiation. PUVA induced apoptosis in leukemia cells one of the ways HL-60 cells to increase Fas gene expression. [Keywords:] photochemical therapy, leukemia, HL-60 cells; K562 cells, NB4 cells; cell apoptosis, Fas Keywords:: PUVA therapy, leukemia, HL-60 cells, K562 cells, NB4 cells, apoptosis, Fas Psoralen (psoralen, PSO) with photosensitive properties and antitumor activity. In this experiment, different concentrations of Singapore length of 360 nm, different time, Ultraviolet irradiation (ultraviolet A, UVA) light effects on human leukemia cells HL-60, K562, NB4, by flow cytometry in the photochemotherapy (PUVA) after apoptosis, ultrastructure changes observed under the electron microscope, fluorescence quantitative PCR and flow cytometry detection of Fas gene and protein expression levels to explore the PUVA method in h