About sinomenine on patients with rheumatoid arthritis fibroblast-like synovial cell proliferation and matrix metalloproteinase -3 expression.docVIP

 PAGE \* MERGEFORMAT 15 About sinomenine on patients with rheumatoid arthritis fibroblast-like synovial cell proliferation and matrix metalloproteinase -3 expression OF: Juan Li Juan Zhao Yi Yu Keqiang [Abstract]: Objective sinomenine in vitro in patients with rheumatoid arthritis fibroblast-like synovial cell proliferation and matrix metalloproteinase -3 (MMP-3) mRNA levels. Methods of cultured human fibroblast-like synovial cells sinomenine into high, medium and low dose group and blank control group, the sinomenine intervention, with the blue tetrazolium cell proliferation was determined by semi-quantitative RT-PCR method for detection of fibroblasts MMP-3 mRNA expression. Results After the intervention of people sinomenine fibroblast-like synovial cell proliferation was significantly inhibited (P lt;0.05), in which the change between the high dose group was particularly significant (P lt;0.01). MMP -3 of the mRNA expression levels compared with the control group decreased significantly (P lt;0.05), and especially the high dose group was significantly different (P lt;0.01). Conclusion Sinomenine patients with rheumatoid arthritis can inhibit fibroblast-like synovial cell proliferation and reduced expression of MMP-3, infer that this may be the treatment of rheumatoid arthritis sinomenine a mechanism. [Keywords:] sinomenine rheumatoid arthritis fibroblast-like synovial cell matrix metalloproteinase -3 Abstract: Objective To observe the effect of sinomenine on the proliferation of fibroblast-like synoviocytes (FLS) and expression level of MMP-3 secreted by FLS in rheumatoid arthritis in vitro. Methods FLS were obtained by digesting synovial tissues with collagens and were divided into four groups: sinomenine high, middle, low concentration and control group. The proliferation of FLS was assessed by methyl-thiazolyl-tetrazolium (MTT) assay and the mRNA expression of MMP-3 was measured by semi-quantitative RT-PCR respectively after treated by sinomen