About two to menstruation particles containing serum on early ovarian granulosa cells in old mice function of.docVIP

 PAGE \* MERGEFORMAT 10 About two to menstruation particles containing serum on early ovarian granulosa cells in old mice function of Of: Wang Lin, and even side, Mafeng Mei, LIU Yan Holland, Sun Zhen high, ZHANG Yan-ping, Teng Wai Hong [Abstract] Objective To observe two to menstruation particles containing serum on the old mouse ovarian granulosa cells in early secretory function in vitro. Methods Serum pharmacological method to the initial drug content of different old serum, and co-cultured granulosa cells cultured 48 h, observed two to menstruation particles containing serum on mouse ovarian granulosa cells in early old estrogen (E2), progesterone (P) secretion (chemiluminescent immunoassay), and inhibin B (INHB) secretion (ELISA method). The results of two to menstruation particles containing serum significantly increased granulosa cell E2, P, INHB secretion. Conclusion of two to menstruation particles can granulosa cells by regulating the secretory function in order to improve ovarian function in early old mice. [Keywords:] two to menstruation particles, ovarian granulosa cells, estrogen, progesterone, inhibin B, serum containing Abstract: Objective To study the effect of serum contained Erzhi Tiangui Granule on the secretion function of ovarian granulosa cells from primary senile mice. Methods The serum with decoction from primary senile mice was added to cultured granulosa cells and incubated for 48 h. Then the concentration of estradiol (E2), progesterone (P) and inhibin B (INHB) in cultured medium were measured by chemiluminescence immunoassay (CLIA) and Sandwich ELISA respectively. Results The serum contained Erzhi Tiangui Granule could increase the secretion of E2, P and INHB. Conclusion Erzhi Tiangui Granule can improve the ovarian function of primary senile mice by regulating the secretion function of granulosa cells. Keywords:: Erzhi Tiangui Granule, ovarian granulosa cell, estradiol, progesterone, inhibin B, serum contained drug