About Injection on limb ischemia reperfusion myeloperoxidase malondialdehyde and enzymes of energy metabolism.docVIP

 PAGE \* MERGEFORMAT 11 About Injection on limb ischemia reperfusion myeloperoxidase malondialdehyde and enzymes of energy metabolism Of: Xie Zhaohui, Li Yan, Wan Yan Hong, Wang Love, Zhou Xiao [Abstract] Objective To observe Injection on limb ischemia reperfusion in skeletal muscle myeloperoxidase (MPO), malondialdehyde (MDA), and cytochrome oxidase (CCO) and the calcium-activated enzyme ATP (Ca2 +-ATPase) activity levels and to explore its mechanism. Methods 30 SD rats, the establishment of limb ischemia model. A group: control group (10), surgery only, not to block , B group: ischemia reperfusion group (10), ischemia and 4 h, and reperfusion 4 h, the absence of drug treatment, C Group: Shenfu treatment group (10), ischemia and reperfusion time with former group. Blocking the femoral artery before the occlusion site of injection Shenfu (2.0 mL / kg), 5 min after clamping the femoral artery and vein. Reperfusion before 15 min, re-injection of the same concentration from the tail vein injection, detection skeletal muscle tissue MPO, MDA, wet / dry weight values #8203;#8203;(W / D) and the CCO and the Ca2 +-ATPase activity. Results Shenfu can reduce skeletal muscle ischemia reperfusion injury in skeletal muscle caused by MPO, MDA, W / D increased, to some extent, protect the CCO and the Ca2 +-ATPase activity. Conclusion Shenfu reduce limb ischemia reperfusion injury in skeletal muscle. [Keywords:] skeletal muscle, reperfusion injury, injection, rats Abstract: Objective To observe the effect of shenfu injection on ischemia / reperfusion injury in skeletal muscle of rats. Methods Rat posterior limb ischemia / reperfusion model were made. Thirty SD rats were randomly divided into three groups (n = 10): sham group ( group A), ischemia / reperfusion group (group B) and shenfu injection treatment group (group C). In group A, the rats received sham operation only. In group B, the animals recieved posterior limb ischemia 4 hours and reperfusion 4 hours. I