Acute carbon monoxide poisoning in rats with delayed encephalopathy after model.docVIP

 PAGE \* MERGEFORMAT 8 Acute carbon monoxide poisoning in rats with delayed encephalopathy after model Authors: ZHAO Tian-chi, Chang Yao-ming, LI Jin-Sheng, Xie Xiaoping [Keywords:] Morris water maze; carbon monoxide poisoning; delayed encephalopathy; rat Establishment of delayed neuropsychologic sequelae rat model after acute carbon monoxide poisoning [Abstract] AIM: To establish the delayed neuropsychologic sequelae rat model after acute carbon monoxide (CO) poisoning for further elucidating its pathogenesis. METHODS: Male SD rats (240-280 g BW) were poisoned with CO in the animal experimental hyperbaric oxygen chamber . Cognitive ability was measured by the escape latency of searching the objective in the Morris water maze. The brains of the rats (3, 7, 10, 20 d after poisoning) were taken for paraffin pathological section and HE staining to observe the pathological change in cerebral cortex and hippocampal area. RESULTS: Rats lost consciousness when they were exposed to 2000 ppm (part per million) CO for 40 min and then 3000 ppm for up to 20 min. Maze test showed that the escape latency of the CO poisoned rats was significantly longer than the air exposed rats (F = 4.74, P = 0.047). The pathological examination revealed apparent neuronal degeneration and necrosis in the cerebral cortex and hippocampal area in CO poisoned rats. CONCLUSION: Besides the evidence of pathologic results, our models basically simulate the praxiologic change induced by central nervous system damage in the patients with delayed neuropsychologic sequelae after CO poisoning. [Keywords] Morris water maze; carbon monoxide poisoning; delayed neuropsychologic sequelae; rats [Abstract] Objective: To establish a rat acute carbon monoxide (CO) poisoning caused by delayed encephalopathy model to explore the pathogenesis. METHODS: Quality of 240 ~ 280 g male SD rats, in animals exposed to hyperbaric oxygen by non-professionals of acute CO , to Morris water maze escape latency in the sear