Semiconservativereplication半保留复制.ppt

Chapter 8 DNA Replication;DNA Replication;8.1 Semi-ConservativeReplication / 半保留复制;8.2 Initiation of Replication / 复制的起始;Bidirectional Replication / 双向复制;8.3 Semi-discontinuousreplication半不连续复制;Semi-discontinuous replication / 半不连续复制;Semi-discontinuous replication / 半不连续复制;8.4 Elongation of Replicationand its Proteins复制延伸及其相关蛋白;8.4.1 Helicase and SSBs / 解旋酶与SSB;3D structure of helicase;SSB: single-strand DNA binding protein;8.4.2 DNA polymerase III / DNA聚合酶III;DNA polymerase III core;Sliding clamp / 滑行夹;Safety clamp for climbing / 攀岩安全扣;3D structure of sliding clamp;Clamp loader / 滑行夹加载器;3D structure of clamp loader;Proofreading / 校正;Proofreading / 校正;Now showingDNA Replication;Now showingDNA polymerization;8.4.3 Explanation for 3’→5’ Synthesis关于3’→5’合成;5’→3’ Synthesis continues after proofreading;5’→3’ Synthesis continues after proofreading;3’→5’ Synthesis stops after proofreading ;3’→5’ Synthesis stops after proofreading ;8.4.4 Primers / 引物;Primer for Okazaki fragment;Primer Removal / 引物去除;8.5 DNA topology / DNA拓扑学;Topoisomerase I / 拓扑异构酶I;Topoisomerase II / 拓扑异构酶II;Termination of replication in E. coli;8.6 DNA Replication in Eukaryotes真核生物DNA复制;Multiple initiation sites多重起始位点;5 eukaryotic DNA polymerases;8.6.1 Replication initiation in Eukaryotes真核生物复制起始;Eukaryotic replication origins真核生物复制起点;The cell cycle / 细胞周期;pre-RC: pre-Replicative Complex前复制复合体;pre-RC: pre-Replicative Complex前复制复合体;Cdk2 and Cdc45;8.6.2 Telomeres / 端粒;Telomeres / 端粒;Telomerase / 端粒酶 (1/2);Telomerase / 端粒酶 (2/2);Telomerase and cancer cell端粒酶与癌细胞;8.7 Experiments / 实验研究;The Meselson-Stahl Experiment ;Experimental evidence;Now showingThe Meselson-Stahl Experiment