After myocardial infarction of bone marrow-derived mesenchymal stem cell therapy to improve cardiac function and cardiopulmonary resuscitation outcome study.docVIP

 PAGE \* MERGEFORMAT 18 After myocardial infarction of bone marrow-derived mesenchymal stem cell therapy to improve cardiac function and cardiopulmonary resuscitation outcome study Author: Wang Tong, Zi-Tong Huang, Fu Yue, direction Shao 【Abstract】 【Objective】 To explore the rat myocardial infarction and after bone marrow mesenchymal stem cells (MSC) therapy, cardiac function changes and their recovery after cardiopulmonary outcomes. 【Methods】 18 male Sprague-Dawley rats were randomly divided into two groups, MSC injection group and the phosphate buffer solution (PBS) injection group, n = 9 rats. First thoracotomy the left anterior descending coronary artery ligation and 4 weeks after re-thoracotomy received PKH26 fluorescent labeled suspended in PBS in the 5 × 106 MSC, or infarction with only PBS injection site, injection after 2 weeks and 4 weeks by echocardiogram Measuring ejection fraction, 4 weeks, induced ventricular fibrillation and conduct cardiopulmonary resuscitation, measurement of cardiopulmonary resuscitation before and 4 h after cardiopulmonary resuscitation hemodynamics within the parameters of change. And 72 h after cardiopulmonary resuscitation myocardial tissue taken for biopsy. 【Results】 The injection compared with phosphate buffer solution, injection MSC 2 Zhou (67% ± 4% vs 56% ± 5%, P lt;0.001) and 4 weeks (67% ± 6% vs 53 ± 13%, P “0.01) and ejection fraction significantly increased before and after cardiopulmonary resuscitation hemodynamic parameters including cardiac index, dp/dt40, -dp/dt, left ventricular end-diastolic pressure was significantly improved; although the success rate of recovery in group 2 no significant difference, but 72 h survival rates of two groups have more significant difference (58.6 ± 20.6 vs 26.1 ± 21.6, P lt;0.05). Myocardial biopsy found a large number of PKH26 labeled cells exist. 【Conclusion】 MSC therapy after myocardial infarction can significantly improve cardiac function, but also can signifi