Angiotensin Ⅱ on umbilical cord blood CD34 cells differentiate into megakaryocytes of.docVIP

 PAGE \* MERGEFORMAT 2 Angiotensin Ⅱ on umbilical cord blood CD34 cells differentiate into megakaryocytes of Authors: DAI Bing, Ji, Chen Shu, Jin-Hui Liu, Fei Qin, Zhu invention, Li-Xing Yan [Abstract] In order to explore the angiotensin Ⅱ on umbilical cord blood CD34 cells induced to differentiate into megakaryocytic cells using MACS (MACS) sorting eight cases of healthy full-term natural delivery of maternal fetal cord blood of the CD34 cells containing platelets TPO (TPO 50 ng / ml), Interleukin -3 (IL-3 10 ng / ml), stem cell-stimulating factor (SCF 50 ng / ml) serum-free culture medium were added to the concentration of 50,100,1 000 μ g / ml of angiotensin Ⅱ as experimental group; the same time in order to not add the basis of angiotensin Ⅱ medium as a control group, cultured for 14 days observation. Cell Counter count the number of mononuclear cells (MNC); flow cytometry counting of CD41 culture system cells, platelet count, and analysis of cell cycle; the use of CD41 monoclonal antibody staining observed in the cell culture system conditions. The results showed that: compared with the control group, experimental group, the number of mononuclear cells, no significant changes (P