Arsenic trioxide combined with Acanthopanax HeLa cell apoptosis induced by experimental study.docVIP

 PAGE \* MERGEFORMAT 17 Arsenic trioxide combined with Acanthopanax HeLa cell apoptosis induced by experimental study 【Key Words】 arsenic trioxide;,, Acanthopanax;,, HeLa cell;, and apoptosis Abstract: Objective To study the arsenic trioxide combined with Acanthopanax inhibitory effects on cancer cells and its mechanism. Method to HeLa cell growth inhibition, flow cytometry PI single-stained cells detected by drug-induced apoptosis. The results of the two drugs combined 24 h group of smaller cells became round and detached conditions are significantly higher than other groups, the group was significantly higher than the rate of cell growth inhibition alone group, combined group of 24 h was significantly higher than the rate of cell apoptosis 16 h and the combined 8 hGroup (P”0.01). Conclusion Ciwujia can be used as anti-cancer arsenic trioxide synergist combination of two drugs has a strong anti-tumor activity, its mechanism and inhibit cancer cell growth, induction of apoptosis. Keywords:: arsenic trioxide; Acanthopanax senticosus; HeLa cells; apoptosis Experimental Study on the Apoptosis of HeLa Cells Induced by Arsenic Trioxide and acanthopanax senticosus Abstract: ObjectiveTo investigate the anti-tumor effect of Arsenic trioxide and Acanthopanax Senticosus on carcinoma cells and its probable mechanism.MethodsHeLa cells were cultured as a pathological model. HeLa cells structural change were examined by inverted phase microscope. The inhibition effect on the growth of cells was evaluated by microculture tetrazolium assay (MTT). The apoptosis of cells were detected by flow cytometry (FCM). ResultsThe cells of the group of two medicines used together turned smaller and rounder than other groups. The growth inhibited rate of this group was higher than that of control group. The apoptosis rate of two medicines used together 24 hours was higher than 16 hours and 8 hours (P lt;0.01). ConclusionAcanthopanax Senticosus can improve the an