Bistorta n-butanol extract on myocardial ischemia.docVIP

 PAGE \* MERGEFORMAT 9 Bistorta n-butanol extract on myocardial ischemia Author: Ye and Yang, Wang Xiurong, Zhi-Hua Huang, Zhou Li, He Wei, Zeng Jing [Keywords:] bistorta n-butanol extract;,, myocardial ischemia;,, coronary artery ligation;,, serum enzymes;,, free radicals Abstract: Objective To observe the boxing reference n-butanol extract of ligation the left anterior descending coronary artery injury in rats. Methods 40 male Wistar rats were randomly divided into sham operation group, coronary artery ligation group, n-butanol extract bistorta high-dose group (120 mg / kg), n-butanol extract bistorta low-dose group (60 mg / kg). The left anterior descending coronary artery ligation myocardial ischemia and continued to order about 120 min, ligation of coronary artery injury model to copy. Records of pre-ischemia, ischemia 15min, administration 30,60,90,120 min Ⅱ -lead ECG, the standard statistical measurement points Ⅱ lead ECG ST-segment deviation Σ ST. The treatment group in the left anterior descending coronary artery ligation front in the duodenum 20 min after injection of bistorta n-butanol extract. Coronary artery ligation group and sham operation group an equal volume of saline injected through the duodenum. End of the experiment after the heart to take blood serum lactate dehydrogenase (LDH), creatine kinase (CK) activity; determination of myocardial tissue malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. On a separate 24 Wister rats with these groups, and animal model-making methods, whichever is to make TTC staining of myocardial infarct size measurements. The results of the treatment group compared with model group, significantly reduced myocardial infarct size, serum LDH, CK activity decreased, serum MDA levels decreased, SOD activity increased, Σ ST the value of reducing, and showed dose-dependent. Conclusion bistorta n-butanol extract treatment group (high, low-dose) on rat coronary artery ligation injury have protective