Bladder cancer tumor suppressor gene Apaf-1 and APC methylation status of its clinical significance.docVIP

 PAGE \* MERGEFORMAT 14 Bladder cancer tumor suppressor gene Apaf-1 and APC methylation status of its clinical significance On: Jun Pan, Chen Lingwu, Wang Wen-Wei, Qu Fu, the sound is king [Abstract] [Objective] The tumor suppressor gene apoptosis protease activating factor -1 (Apaf-1) and adenomatous polyposis coli gene (APC) promoter methylation status and the relationship between bladder cancer and clinical pathology the impact on relapse. [Methods] methylation specific PCR, 110 patients with bladder cancer and APC in the Apaf-1 promoter methylation status of 15 cases of normal bladder tissue for the control group. [Results ] Apaf-1 and APC genes in bladder cancer tissue methylation rate was 90.0% and 82.7% in normal bladder tissue was 6.77% and 20.0%, respectively, statistically significant differences (P all = 0.000). Apaf- 1 and APC methylation rate and degree of tumor differentiation and clinical stage significantly correlated with tumor grade, increased with increasing stage. in the recurrence group and the comparison group without recurrence, Apaf-1 methylation-positive rates were 97.6% (41/42) and 85.3% (58/68), the difference was statistically significant (2 = 4.382, P = 0.036), and APC methylation rates were 85.7% ( 36/42) and 80.9% (55/68), the difference was not statistically significant (2 = 0.424, P = 0.515). [Conclusion] Apaf-1 and APC methylation changes in bladder cancer, the development process play an important role, Apaf-1 promoter methylation may be prognostic in patients with bladder cancer molecular markers. [Keywords:] bladder cancer; methylation; tumor suppressor gene; Apaf-1; APC Abstract: [Objective] To study the Apaf-1 and APC promoter methylation status and its relation with clinical pathology and prognosis of bladder carcinoma. [Methods] Methylation status of Apaf-1 and APC gene promoter in 110 specimens of bladder carcinoma and 15 normal bladder tissues were detected by methylation-specific PCR. [Results] The methyla