Bone marrow mesenchymal stem cells combined with sound hedgehog factor - collagen-based nanocrystalline repair of bone defects.docVIP

 PAGE \* MERGEFORMAT 17 Bone marrow mesenchymal stem cells combined with sound hedgehog factor / collagen-based nanocrystalline repair of bone defects Of: history of strong Sunguang You, Ma Peng, Qian Dong Xu Xiaofeng Xu Chengzhen [Abstract] Objective: To investigate the bone marrow mesenchymal stem cells (marrow mesenchymal stem cells combined tone hedgehog factor (sonic hedgehog, Shh modified collagen-based bone nanocrystalline (nano Hydroxyapatite / collagen, nHAC) repair of femoral defects in rats. Methods: SD rat bone induction by the MSCs grown on scaffold-forming cells scaffold. SD 30 rats into 5 mm segmental femoral defect, divided into control group: the bone defect implants do not plant , MSCs / nHAC groups: implanted MSCs / nHAC composites, MSCs / Shh nHAC groups: implanted MSCs / Shh nHAC complex. by gross observation, X ray radiography, histology, bone defects in each group compared. Results: SD rat MSCs in vitro and nHAC, Shh nHAC composite culture day 7, SEM confirmed that Shh nHAC more conducive to MSCs adhesion and proliferation. postoperatively 6,12 weeks radiological evaluation of new bone formation was statistically different between the groups significance (P lt;0.05). Postoperative histological examination of each group of new bone formation rate, formation significantly (P lt;0.05). CONCLUSION: MSCs / Shh nHAC complex SD rat femoral defect can be repaired, repair better than MSCs / nHAC complex. [Keywords:] bone marrow mesenchymal stem cells; nanocrystalline bone collagen; bone defect; tone hedgehog factor [Abstract] Objective: To explore the repair effect of the segmental bone defects in rats with nano Hydroxyapatite / collagen modified by bone marrow mesenchymal stem cells co cultured with sonic hedgehog (Shh.Methods: MSCs obtained from SD rats bone marrow were induced and proliferated . After their osteoblastic phenotype had been demonstrated, MSCs were seeded onto the scaffolds.A bone defect (5 mm in length) was created in