Bromothymol blue colorimetric determination of alkaloid content of Phyllanthus.docVIP

 PAGE \* MERGEFORMAT 9 Bromothymol blue colorimetric determination of alkaloid content of Phyllanthus Author: Tang Guanghui Hu Wei Liu Fuzhou Zhang Guihua Agriculture, Forestry and [Abstract] Objective To determine the total alkaloid of Phyllanthus content. Methods bromothymol blue as chromogenic reagent was measured at 415 nm wavelength absorbance. The results of matrine in the 0.004 ~ 0.02 mg / ml range a good linear relationship (r = 0.997 54); with bromothymol blue ion pair formed by the product was stable in 6 h; the average recovery was 98.65% (RSD = 1.98%). Conclusion The method