Chloride channel blockers on the rat hippocampal neurons in vitro apoptosis of.docVIP

 PAGE \* MERGEFORMAT 9 Chloride channel blockers on the rat hippocampal neurons in vitro apoptosis of Study: Chang Shu-Ling Zhang Cai Shining Yin Jinbao Complete Loyalty [Abstract] Objective To observe the chloride ion (Cl-channel blockers on nitric oxide (NO donor 3 morpholine de olamine (SIN 1-induced apoptosis of hippocampal neurons in vitro the effect of Cl- channels in ischemic brain injury. Methods 12 d in vitro culture of SD rat hippocampal neurons were divided into normal control group, SIN 1 treatment group, SIN 1, and after Cl-channel blocker group to neurons in each group at the corresponding time points Hoechst fluorescence staining experiments apoptotic cells and quantitative analysis of MTT survival of neurons. Results SIN 1 significantly induced neuronal apoptosis (P lt;0.05, SITS and DIDS in a dose dependently inhibited the NO-induced neuronal injury and improve the survival rate of neurons with SIN 1 group difference was significant (P lt;0.05. Conclusion Cl-channel blockers on NO-induced apoptosis in rat hippocampal neurons a protective effect. [Keywords:] chloride ion channel, NO, hippocampus, rat, apoptosis [Abstract] Objective To observe the effects of two kinds of chloride channel blockers SITS and DIDS on the hippocampal neuronal damage induced by NO in culture and explore the roles of chlorides in the cerebral ischemic injury. Methods The cultures were divided into 3 groups: control , NO treatment, NO treatment plus chloride channel blocker groups. The cultures were detected with the methods of morphological stain (Hoechst 33258) and MTT quantitative analysis to observe the apoptotic neurons and neuronal viabilities. Results There was a significant protective effects of SITS and DIDS on neuronal damage with dose dependence. Conclusions Chloride channel blockers have some protective effects against neuronal injury induced by NO. [Keywords:] Chloride channel; NO; Hippocampus; Rat; Apoptosis Death of ischemic neurons in th