Clinical isolates of Acinetobacter baumannii resistance genes abeM Research.docVIP

 PAGE \* MERGEFORMAT 19 Clinical isolates of Acinetobacter baumannii resistance genes abeM Research Author: Su-Hin-room-ting Shen Feng Deng households 【Abstract】 Objective To investigate the clinical isolates of Acinetobacter baumannii (Acinetobacter baumannii, Ab) the molecular mechanism of drug resistance. Methods in clinical isolates of Ab 35, Ab 36 chromosomal DNA as a template amplified abeM gene pairs abeM gene amplification products, DNA sequencing and homology analysis; abeM gene amplification products will be connected with the vector transformed into Escherichia coli bacteria KAM32 competent cells, the right to conduct sensitivity analysis of transformants. The results of Ab 35 and Ab 36 in abeM gene amplification products with the reported nucleotide sequence homology abeM respectively 99% and 98%, Ab 35’s abeM structural gene deduced amino acid sequence occurred in three amino acid residues mutation, Ab 36 of the two amino acid residues mutated; containing Ab 36 of the abeM gene transformants with a broader and stronger resistance map; two kinds of transformants can be drug resistance efflux pump inhibitor CCCP and verapamil reversed. Conclusions Acinetobacter baumannii AbeM drug efflux protein, amino acid residues Val 52, Gln 203 and Ser 256 of multiple drug-resistant strength of an important role in drug efflux pump inhibitors can effectively inhibit AbeM resistance protein activity. Keywords: multi-drug resistance; gene cloning; Acinetobacter baumannii ABSTRACT Objective To investigate the molecular mechanism of drug  resistance in Acinetobacter baumannii (Ab) clinical isolates. Methods PCR amplification of drug resistance gene abeM was carried out using chromosomal DNA of Ab 35 and Ab 36 clinical isolates as template. PCR product of abeM gene was sequenced. The nucleotide sequences and deduced amino acid sequences were analyzed by BLAST of NCBI. After cells of Escherichia coli KAM32 transformed with a plasmid carrying