Cmyc gene and P16 gene expression in colon cancer and its clinical significance.docVIP

 PAGE \* MERGEFORMAT 6 Cmyc gene and P16 gene expression in colon cancer and its clinical significance [Keywords:] colon, C myc gene, P16 gene In the course of the development of malignant tumors, oncogene activation and (or) inactivation of tumor suppressor genes play an important role in colon cancer and evolution of the same variety of molecular events associated with the occurrence of (1). The original C myc oncogene can make unlimited cell proliferation, have eternal life, and can promote cell division, and the occurrence and development of many tumors (2,3). The P16 gene is an important tumor suppressor gene, directly involved in cell cycle regulation negative regulation of cell proliferation and division. We examined 60 cases of colon cancer patients C myc gene and P16 gene expression, and its clinical significance. 1 Materials and methods 1.1 study Collected from March 2003 to September 2007 our department of colon cancer resection specimens of 60 patients, including 45 males and 15 females, aged 35 to 86 (mean 67 years. All cases were diagnosed by histological , no preoperative radiotherapy and chemotherapy. histologic grade of primary tumor: 45 cases of highly differentiated, poorly differentiated in 15 cases. tumor stage: 5 cases, 12 cases, of 25 cases, of 18 cases. metastasis: lymph node metastasis in 35 cases, 25 cases without lymph node metastasis. 40 cases from the tumor margingt; 5 cm, pathological examination was normal adjacent tissues as the control group. 1.2 Reagents and Methods C myc gene and P16 gene in monoclonal antibodies and immunohistochemistry (SABC method kits were purchased from California Biotech Inc company. Immunohistochemistry operation according to kit instructions. PBS instead of first antibody as negative control, a known liver organization as a positive control. 1.3 results found Nucleus and / or cytoplasm showed a brown reaction is positive. Positive cells: biopsy specimens were selected for