Conditions of Con A in vitro culture of rabbit corneal endothelial cell growth.docVIP

 PAGE \* MERGEFORMAT 19 Conditions of Con A in vitro culture of rabbit corneal endothelial cell growth [Abstract] Objective concanavalin A (concanavalin A, ConA) conditioned medium on cultured rabbit corneal endothelial cells (rabbit corneal endothelial cells, RCECs) growth. Methods exposing Descemet taken and endothelial cells in the United RCECs tissue in vitro method for primary culture were used to contain the volume fraction of 5%, 10%, 15%, 20% ConA activated spleen cell conditioned supernatant of RPMI 1640 medium RCECs primary culture, and with 10% (by volume) fetal bovine serum (fetal bovine serum, FBS) in RPMI 1640 medium served as controls. neural enolase immunohistochemistry staining cells were identified, MTT colorimetric growth observed in each group RCECs conditions, SPSS11.0 One-way ANOVA and LSD test for statistical analysis. RCECs was successfully carried out in vitro, cultured cells expression of neural enolase. ConA conditioned medium for cell proliferation in each group were higher ( P lt;0.001), and 10%, 15% ConA conditioned medium group RCECs cells was significantly higher than the other experimental groups (P lt;0.001). Conclusions ConA conditioned medium could promote the growth of RCECs can be used as training adjuvant agents. [Keywords:] corneal endothelial cells, primary culture, concanavalin A, MTT, neural enolase ABSTRACT: Objective To evaluate the effect of conditioned medium made up of ConA-activated spleen cells supernatant on cultured rabbit corneal endothelial cells (RCECs). Methods RCECs were primarily cultured in vitro by revealing descemet membrane and endothelium combining tissues masses. Culture medium was RPMI 1640 with 10% FBS and varied concentrations of conA-activated spleen cells supernatant. Experimental groups were divided by different concentrations of ConA-conditioned supernatant: 5%, 10%, 15%, and 20%. In the control group only RPMI1640 and 10 % FBS were used. Methyl thiazolyl tetrazolium colorimetry ana