Containing anti-apoptosis gene bcl -2 recombinant retrovirus recombinant and identification of.docVIP

 PAGE \* MERGEFORMAT 11 Containing anti-apoptosis gene bcl -2 recombinant retrovirus recombinant and identification of 【Abstract】 Objective: To construct and identify the mouse bcl -2 gene-containing recombinant retrovirus. Methods: The anti-apoptosis gene bcl -2 fragment from the vector pcDNA3.1 in the cut, and directed into retroviral vector PLNCX2, the restriction enzyme digestion; liposome into the retroviral packaging cell line PT67, G418 Screening to establish stable cell lines expressing bcl -2. Results: The double-enzyme digestion, the success of recombinant retroviral vector; recombinant retrovirus into packaging cell PT67, after G418 selection, resistant colonies formed, and measured the virus titer was 3 × 1011cfu / L, prompt construction containing mouse bcl -2 gene recombinant retroviral vector and stable packaging cell line successfully. Conclusion: The anti-apoptosis gene bcl -2 containing recombinant retroviral vector was successfully constructed. Keywords: recombinant retroviral vector gene bcl -2 0 Introduction With some of the common blinding eye disease prevention and control measures have been better, refractory or non-government eye disease has become an eye research hot spot and focus. Refractory eye disease involving mainly refers to the number of retinal degenerative diseases, such as hereditary retinal degeneration, age-related macular degeneration, retinal pigment epithelium (RPE), and cone-rod (photoreceptor) cells of the progressive degeneration and death, yet so far No to prevent the degeneration and death of these cells in an effective manner. Us to build anti-apoptosis gene bcl -2 recombinant retrovirus to import it a normal SD rats were cultured RPE cells, then micro-injected into mice with retinitis pigmentosa subretinal space of retinal degenerative disease genes Treatment of conduct exploratory research, in order to construct and identification of bcl -2 gene recombinant retroviral coverage of work