Cornel Polysaccharide Extraction and Characterization of.doc

 PAGE \* MERGEFORMAT 9 Cornel Polysaccharide Extraction and Characterization of Authors: ZHAO Xiao-hua, Wang Yi-fei, Liu Qiuying, An Wei Zheng [Abstract] through a single factor and orthogonal experiment to polysaccharide yield as an indicator to determine the optimal extraction process of polysaccharide dogwood, while Cornel Polysaccharide analysis of physical and chemical properties, results show that the optimum water extraction process of polysaccharide Cornus conditions: temperature 80 ℃ , solid-liquid ratio 1:60, time 5 h, in this condition dogwood rate is 13.8% polysaccharides by DEAE-52 cellulose ion-exchange and SephadexG-200 column chromatography to be components of S21, was measured by elemental analysis C7.80%, H% 5.64%, S% 0.37%, N not detected. [Keywords:] Cornus polysaccharide; extraction; isolation and purification; physical and chemical properties Traditional Chinese medicine for the Cornaceae Cornus Dogwood Cornus officinalis Sieb.et Zuce plant dry ripe fruit, mainly in Zhejiang, Anhui, Henan and other places. Its taste Suanse, sexual tepid, go through the liver and kidney, with liver and kidney tonic convergence Guse role. Modern pharmacological studies have shown that dogwood fruit and the stones are rich in carbohydrates and various amino acids, VitB1, VitB12, etc. [1]. Cornus officinalis polysaccharide immune regulating activity and significant anti-oxidation [2,3] as China’s socio-economic and cultural development, people’s quality of life generally improved, so has the function of preventive health care are increasingly attracted people’s attention dogwood. In this experiment, polysaccharide yield as an indicator, using orthogonal pairs of Cornus to optimizing extraction process of polysaccharide with a view to the preparation process to identify reasonable and practicable and to study and analyze some of its physical and chemical properties. An apparatus and method 1.1 Materials Cornus tested dry fruit produced in Anhui, purchas