D-limonene on the K562 cell proliferation and apoptosis of.docVIP

 PAGE \* MERGEFORMAT 15 D-limonene on the K562 cell proliferation and apoptosis of Abstract This study investigated the D-limonene on the K562 leukemia cell proliferation and its mechanism. Application of MTT test the effects of different concentrations of D-limonene After the effects of changes in K562 cells; application of cell morphology, flow cytometry, DNA agarose gel electrophoresis and detection of apoptosis. The results showed that: In the 0.125-1.0 mmol / L concentration range, D-limonene on the K562 cell proliferation in a dose-dependent relationship. A typical cell morphological changes, DNA ladder by agarose gel electrophoresis and flow cytometry to detect sub-G1 peak confirmed the common D-limonene can induce apoptosis of K562 leukemia cells. Conclusion: D-limonene inhibit proliferation of K562 cells and a dose-dependent manner, so that cells remain in G1 phase and induce their apoptosis. Keywords: D-limonene; K562 cells; apoptosis; cell proliferation Proliferation Inhibition and Apoptosis Induction of K562 Cells by D-limonene AbstractThis study was aimed to investigate the effect of D-limonene on K562 leukemia cells and its mechanism. Inhibitory effect of D-limonene on proliferation of K562 leukemia cells was assayed by MTT method and cell apoptosis was detected by flow cytometry and DNA agarose gel electrophoresis , the morphologic change of K562 cells was observed by microscopy. The results showed that when K562 cells were treated with 0.125-1.0 mmol / L of D-limonene for 48 hours, the proliferation of K562 cells was obviously inhibited in dose-dependent manner. Typical morphological changes and the typical DNA ladder on agarose gel electrophoresis for analysis of cellular apoptosis were significantly appeared in D-limonene treated K562 cells. Simultaneously, the sub-G1 peak was found in FCM analysis. It is concluded that the D-limonene can inhibit proliferation of K562 cells in dose-dependent manner, cause cell detai