Delta Module HLA typing method for detection of.docVIP

 PAGE \* MERGEFORMAT 14 Delta Module HLA typing method for detection of Author: Lu Qin-Feng, Zhang Wei, Zhu invention, Li-Xing Yan 【Abstract】 In order to establish delta block matching-based detection methods, using salting out extraction of DNA, PCR amplification delta module, GeneScan mode detection PCR. The results showed that in 104 random samples, PCR amplification of the delta module has a high degree of polymorphism. DNA fragment length range of 81-393 bp, the number of fragments 6-32 months; fragment length concentrated in the 81-118 bp ,140-175 bp ,217-301 bp ,340-393 bp 4 regions. Conclusion: The delta block matching technique is feasible, can be used for hematopoietic stem cell transplantation donor screening. The first time a Chinese Han population delta module data. Keywords: delta Module Establishment of Delta Block Matching Technique Abstract To establish delta block HLA-matching technique, DNA was extracted from whole blood by salting-out method, delta block was amplified by polymerase chain reaction (PCR), and PCR product was detected by GeneScan.The results showed that delta block had polymorphism in 104 samples without sibship of the Han people from Zhejiang province.The range of DNA fragment length was 81-393 bp and could be divided into 4 groups: 81-118 bp ,140-175 bp ,217-301 bp ,340-393 bp.The numbers of DNA fragments were 6-32. It is concluded that the method of delta block matching is reliable and can be applied to select donors for the patients to be transplanted.It is the first time to get delta block data of the Han people in China. Keywords: delta block; delta block matching; HLA-matching technique; block matching Block matching technique (block matching) in hematopoietic stem cell transplantation has a certain significance. Witt et al [1] The study found that hematopoietic stem cell transplantation for the recipient HLA-A, B, and DRB 6 loci coincide, the transplant patients affected by