Dermatomyositis patients with CD4 + T cells expression and significance of TGF1mRNA.docVIP

 PAGE \* MERGEFORMAT 11 Dermatomyositis patients with CD4 + T cells expression and significance of TGF1mRNA [Abstract] Objective: To detect the activity of dermatomyositis (dermatomyositis, DM) patients with CD4 + T cells in peripheral blood of transforming growth factor 1 (transforming growth factor 1, TGF 1 and serum creatine kinase (CK content. Preliminary study CD4 + T cells in the pathogenesis of TGF 1 in the role of dermatomyositis. Methods: 12 patients with active dermatomyositis patients and 12 healthy controls, peripheral blood, using immunomagnetic bead separation of CD4 + T cells obtained by RT PCR method detect the expression of TGF 1 in each group. observed in CD4 + T cells the expression of TGF 1 correlated with the CK content. Results: The peripheral blood of dermatomyositis patients CD4 + T cells, the relative expression of TGF 1 was 0.751 + -0.107 , significantly higher than in healthy controls 0.438 + -0.170 (P lt;0.05). CD4 + T cells in TGF 1 expression and CK levels were moderately correlated (r = 0.67, P lt;0.05). Conclusion: Patients with active dermatomyositis peripheral blood CD4 + T cells in TGF 1 mRNA expression was significantly increased occurrence of disease may be associated with dermatomyositis. [Keywords:] CD4 T cell 1 dermatomyositis [Abstract] Objective: To investigate the expression of trans forming growth factor 1 (TGF 1) mRNA in CD4 + T cells of peripheral blood from the dermatomyositis (DM) patients in active stage, and explore the association with serum creatinkinase levels. Methods: CD4 + T cells from peripheral blood were isolated by microbeads, and TGF 1 mRNA was measured by reverse transcriptase polymerase chain reaction (RT PCR). Results: The expression of TGF 1 was significantly higher in CD4 + T cells from the dermatomyositis patients than those from healthy control group (0.751 + -0.107 vs 0.438 + -0.170, P lt;0.05). There was positive correlation between the expression levels of TGF 1 mRNA and serum creat