Determination of hemoglobin oxygen carriers in the residual glutaraldehyde.docVIP

 PAGE \* MERGEFORMAT 15 Determination of hemoglobin oxygen carriers in the residual glutaraldehyde Study: Small Dan Han Jing Jing Yan Kunping but rather Chen Chao [Abstract] established a method for determining the residual glutaraldehyde hemoglobin oxygen carriers in the HPLC method. With a 10 kDa ultrafiltration membrane by centrifugation 3000 r / min × 20 min to free glutaraldehyde, separated from the sample ; at pH 1.0, in the system containing 70% acetonitrile using 2,4-dinitrophenylhydrazine in 30 min, the glutaraldehyde Brown List 2,4-dinitrophenyl hydrazone (n (2,4-dinitro phenylhydrazine): n (GA) = 60:1) to 70% acetonitrile as mobile phase, to avoid precipitate formation of derivative products. by high performance liquid chromatography separation and determination of derivatives can be separated within 20 min, At the same time steps were optimized derivative. This method has high sensitivity and good linear relationship between the detection limit of 1.0 ng, in the 0.1 ~ 10 mg / L range and the linear correlation coefficient of 0.9999, repeated measurements 6 The relative standard deviation less than 3.0%, recovery was 95.26%. [Keywords:] glutaraldehyde, HPLC, hemoglobin oxygen carrier, 2,4-dinitrophenylhydrazine 1 Introduction Hemoglobin oxygen carrier (Hemoglobin based oxygen carriers, HBOCs) are blood substitutes one of the hot research field [1,2],. The preparation method is commonly used as crosslinker more aldehyde molecules, such as glutaraldehyde, such as the open-loop raffinose cross-linked hemoglobin prepared from [3,4]. free of glutaraldehyde with some toxicity, must be removed before use in clinical practice. Detection of aldehydes by gas chromatography [5 ~ 7] have a high analysis speed, but relatively low sensitivity [8]. Performance liquid chromatography is widely used in the determination of aldehydes [9, 11], in the determination of glutaraldehyde, the show a higher sensitivity [8]. In liquid chromatography, the 2,