Different areas in Guangxi southern honeysuckle determination of chlorogenic acid.docVIP

 PAGE \* MERGEFORMAT 8 Different areas in Guangxi southern honeysuckle determination of chlorogenic acid [Abstract] Objective To determine the different areas of Guangxi in southern China chlorogenic acid content of honeysuckle, Lonicera medicine evaluation of the quality of South China. Methods HPLC, Angilent ODS C18 (4.6 mm × 150 mm, 5 μm) column with acetonitrile - methanol - 0.05% phosphoric acid as the mobile phase gradient elution, the ratio of three-phase 0 ~ 20 min to 13:13:74 for the 7:5:88,25 min flow rate of 0.5 mL / min, detection wavelength was 327 nm, column temperature is 30 ℃. The results from different areas of Guangxi in southern China Honeysuckle chlorogenic acid content range 2.21% ~ 3.94%. Conclusions from different areas of Guangxi in southern China Honeysuckle chlorogenic acid there are some differences, but have reached lt;lt;Chinese Pharmacopoeia gt;gt; requirements. [Keywords:] South honeysuckle, chlorogenic acid, HPLC, determination Abstract: Objective To determine the content of chlorogenic acid in Fructus Lonicera Confusa DC (FLCD) from different places in Guangxi by HPLC, and evaluate its internal quality. Methods HPLC separation was performed on a Agilent C18 analytical column (4.6 mm × 150 mm, 5 μm) by gradient eluting with acetonitrile-methanol-0.05% phosphoric acid at the flow rate of 0.5 mL / min. The column temperature was 30 ℃ and the UV detection wavelength was 327 nm. Results The chlorogenic acid content of FLCD from different places in Guangxi ranged from 2.21% to 3.94%. Conclusion The chlorogenic acid contents of FLCD from different places in Guangxi are different, but all of them are up to the standard of Phamarcopoeia of the People’s Republic of China. Keywords:: Fructus Lonicera Confusa DC, chlorogenic acid, HPLC, content determination South honeysuckle is Lonicera confusa A, the Department of South honeysuckle Caprifoliaceae Lonicera Lonicera confusa DC.’s Dried flower buds, with the effect of cleari