Eclipta prostrata Extraction of flavonoids and their antioxidant activity in vitro study of.docVIP

 PAGE \* MERGEFORMAT 15 Eclipta prostrata Extraction of flavonoids and their antioxidant activity in vitro study of Author: Wang Xuemei Zhang Jiansheng Dai Yun Gao Yun-tao [Abstract] Objective To study the Eclipta prostrata Extraction of flavonoids and their antioxidant activity. Methods Fentaon reaction to produce hydroxyl radical OH, illumination of riboflavin produced superoxide anion radical O-2 * , using spectrophotometry Eclipta prostrata in vitro the role of oxygen free radical scavenging activity, with thiobarbituric acid (TBA) was studied by spectrophotometry of its OH-induced lecithin lipid peroxidation and DNA oxidative damage in vivo. Results Eclipta prostrata yield of total flavonoids was 3.96%, and OH, O-2 * half-clearance IC50 of 0.015 mg * ml-1, 0.08 mg * ml-1; pairs of DNA oxidative damage of lipid peroxidation in the semi - inhibition rate was 0.02 mg * ml-1, 2.0 μ g * ml-1. Conclusion Eclipta prostrata flavonoids can effectively remove active oxygen free radicals and lipid peroxidation of lecithin, and DNA oxidative damage in a significant inhibition. [Keywords:] Eclipta prostrata; total flavonoids; anti-oxidation Abstract: ObjectiveTo study the extraction of flavone from Eclipta alba and its antioxidation function.MethodsScavenging effects of Eclipta alba on active oxygen species * OH and O * -2 generated by Fenton reaction and riboflavine photosensitization were investigated by the means of spectrophotometry. The inhibitory effects of Eclipta alba on the lecithin lipid peroxidation and damage of DNA chain induced by hydroxyl radical were observed by thiobarbit uric acid spectrophotometry.ResultsTotal flavonoids in Eclipta alba was 3.96%; the semi-clearance rate of * OH and O * -2was 0.015 mg * ml-1 and 0.08 mg * ml-1; the semi-inhibition rate of Lecithin lipid peroxidation and oxidation damage of DNA was 0.02 mg * ml-1 and 2.0 μ g * ml-1.ConclusionEclipta alba can scavenge active oxygen efficiently. It can also significantly