Effect of verapamil on LPS-induced apoptosis in rat lymphocytes and the pro-inflammatory - anti-inflammatory cytokines influence the proportion of.docVIP

 PAGE \* MERGEFORMAT 4 Effect of verapamil on LPS-induced apoptosis in rat lymphocytes and the pro-inflammatory / anti-inflammatory cytokines influence the proportion of Author: Wang Li Gang Qi Xiaoping Jieshou [Keywords:] Endotoxin verapamil lymphocyte apoptosis factor 0 Introduction Recent studies found that verapamil to control lipopolysaccharide (LPS)-induced local and systemic pro-inflammatory / anti-inflammatory cytokine expression, and can reduce mortality in experimental animals [1-2]. Sepsis pro-inflammatory / anti-inflammatory cytokines and the relationship between apoptosis of lymphocytes is very complex and verapamil on circulating lymphocyte apoptosis, pro-inflammatory / anti-inflammatory cytokine balance in vivo is sparse. Therefore, this Study aims to to explore the effects of verapamil on LPS-induced pro-inflammatory / anti-inflammatory cytokine balance, lymphocyte apoptosis, and its circulating pro-inflammatory / anti-inflammatory cytokines in the relationship between the ratio and lymphocyte apoptosis. 1 Materials and methods 1.1 Materials adult male SD rats (body weight 250 ~ 300 g) from Nanjing General Hospital of Nanjing Military Region Experimental Animal Center of the experimental pre-fasting 8 h. LPS, verapamil, liquid Histopaque 1.077 density gradient centrifugation were purchased in the United States Sigma Corporation; apoptosis kit purchased from Nanjing Checchi Biotechnology Development Corporation; TNF  α , IL  6 and IL  10 ELISA kit were purchased in the United States Bio  source company. 1.2 Methods 1.2.1 Grouping and treatment of 42 rats were randomly divided into 7 groups: control group (A); LPS group (B); (C ~ F) of different doses of verapamil group; verapamil group ( G). C ~ F group rats were given 1, 2.5, 5, 10 mg / kg intraperitoneal injection 30 min after verapamil; B ~ F group rats were given intraperitoneal injection of LPS (10 mg / kg, 1 μ L / g), LPS with 90 mL / L sterile saline configured as 1 mL / L