Emei production flavor with fingerprint.docVIP

 PAGE \* MERGEFORMAT 8 Emei production flavor with fingerprint Author: Jiang Triangle, Machao Ying, Liu Jun, Lin Lin, Wu Xiaoqing [Keywords:] Emei taste even the middle class; fingerprinting; high-performance liquid chromatography Abstract: ObjectiveTo establish a sensitive and specific HPLC method for the quality control of Emei’s Coptis chinensis Franch.MethodsThe chromatographic fingerprint of Emei’s Coptis chinensis Franch was obtained by RP-HPLC (DAD) and the gradient elution mode was applied in chromatographic separation, data was analyzed by Fingerprint Similarity Evaluation Software to compare the similarity of samples.ResultsThe HPLC fingerprint of Emei’s Coptis chinensis Franch was established preliminarily, showing 9 characteristic peaks, by comparision of the retention time of chemical standards, peak 6,8 and 9 were identified as Jatrorrhizine , Palmatine and Berberine.ConclusionThe HPLC fingerprint method is repeatable and can be used for quality control of Emei’s Coptis chinensis Franch. Keywords:: Emei’s Coptis chinensis Franch; Fingerprint; HPLC Emei in Sichuan produced well-known authentic flavor with herbs, plants from Ranunculaceae Coptis Coptis chinensis Franch (Xi-called ‘taste even’) of the roots in autumn excavation, removal of fibrous roots and sediment, dry, remove residual fibrous root [1] . Bitter, cold, and heart, liver, stomach, large intestine through, with heat dampness, Xiehuo detoxification function, is a clinical application of a wide range of heat-clearing and dampness antidote commonly used in hot and humid or fullness, vomiting, Xie Li , jaundice, fever Shenhun, Firelight Kang-sheng, toothache, Diabetes, and carbuncle swollen boils; outside the governance of eczema, wet sores, discharging ear canal, middle ear swelling and ulcers and other diseases [2 ~ 4]. Taste even the main component of rhizome jatrorrhizine (jatrorrhizine), coptisine (coptisine), THP (palmatine), berberine (berberine), pseudo-berberine (epiberberi