Enhanced capillary zone electrophoresis sample midfield nonlinear characteristics of enrichment column.docVIP

 PAGE \* MERGEFORMAT 17 Enhanced capillary zone electrophoresis sample midfield nonlinear characteristics of enrichment column [Abstract] sample direct field effect is a stigma capillary zone electrophoresis column enrichment, the process of its sample distribution of the sample in the column can be divided into two parts, namely, the accumulation in the running buffer zone and by electroosmotic flow section of the introduction of the sample solution. Through the solute transport behavior research shows that: the length of the two sections between the injection time is not a simple linear relationship, so the injection volume and injection time, non-linear relationship , and with the solute mobility is related to the increase in sample size does not lead to the enrichment factor of the synchronous increase in the role of the laminar flow into the sample column makes field-effect enrichment reduced. In order to remain basically unchanged under the column efficiency get good enrichment, in addition to the solute in the running buffer and the electrical conductivity of the sample solution than the great, the injection time should match. [Keywords:] capillary electrophoresis, concentration, injection Abstract FASS (field amplified sample stacking) is a technique for on column enrichment in capillary zone electrophoresis. The distribution of solute in column during injection can be divided into two parts: the stack zone in running buffer and the sample solution zone imported by electroosmosis. Based on the investigation of transfer of solute in column, the results showed that the relationship between the length of two zone and injection time was nonlinear, so the relationship of injecting size that related with the mobility of solute and injection time. The increasing of the enrichment effect will not synchronize with the increasing of injection size, and the enrichment effect is decreased due to the laminar flow. To hold a basic constant column efficiency