Five new photosensitizer two methods of lysine carbonyl2zinc phthalocyanine and Bovine Serum Albumin.docVIP

 PAGE \* MERGEFORMAT 18 Five new photosensitizer two methods of lysine carbonyl2zinc phthalocyanine and Bovine Serum Albumin Of: Chun-Yan Li, Ruan Guanyu, single Li, Chen Jincan, Huang Mingdong [Abstract] Objective 2 new photosensitizer carbonyl five lysine zinc phthalocyanine (ZnPc (Lys) 5 and bovine serum albumin (BSA interaction. Method from the point of BSA by fluorescence spectroscopy and UV-visible spectroscopy by fluorescence quenching experiments of ZnPc (Lys) 5 and the quenching mechanism of interaction between BSA, the binding constant and binding sites, from the ZnPc (Lys) 5 point micellar fluorescence enhancement assay for ZnPc (Lys) 5 concentrations were determined by Scatchard equation ZnPc (Lys) 5 and the interaction between BSA binding constants and binding sites. The results show that the fluorescence quenching results ZnPc (Lys) 5 BSA has a strong fluorescence quenching, quenching mechanism is static quenching the formation of ground state complexes. different temperatures (298,303,308,313,318 K the binding constant K1 was 12.1 104 L / mol, 7.69 104 L / mol, 6.12 104 L / mol, 4.57 104 L / mol, 3.76 104 L / mol number of binding sites were 0.93,1.02,1.07,1.13,1.17. Scatchard equation to calculate the binding constant K2 298 K for the 1.557 105 L / mol, binding sites-bit 1.07. Conclusion calculated from the two binding constants and binding basically the same loci, ZnPc (Lys) 5 and the formation of serum albumin between the ground state mainly by way of the complex interaction of serum albumin into a binding site. [Keywords:] light-sensitive drugs, spectrometry, fluorescence, spectrophotometry, ultraviolet light, zinc, lysine, indole, serum albumin, bovine Interaction of Pentalysine carbonyl phthalocyanine Zinc with Bovine Serum Albumin: Two Methods LI Chunyan, RUAN Guanyu, SHAN Li, CHEN Jincan, HUANG Mingdong School of Pharmacy, Fujian Medical University, Fuzhou 350004, China; Department of pharmacy, Fujian Maternal an