Gastric SOX4 mutation analysis.docVIP

 PAGE \* MERGEFORMAT 14 Gastric SOX4 mutation analysis Author: Wang Fang, Ge Yadong, NIE Liu-wang [Abstract] Objective To study the gastric SOX4 gene and its encoded amino acid base mutation and explore its role in gastric cancer. To extract gastric cancer and adjacent tissues of the DNA, PCR amplification of gastric cancer SOX4 gene HMG-box frame, and its conduct PCR-SSCP analysis, there are differences, DNA sequencing samples. The sequencing results with the ClustalX software with the normal HMG-box frame sequence alignment, using DNAStar software HMG-box frame base sequence into amino acid sequence, and then ClustalX software with the normal amino acid sequence alignment right. Results sequencing was found in 27 cases of gastric cancer samples, 12 patients had point mutations, and in the adjacent tissues did not find mutations in advanced gastric cancer was significantly higher than the mutation rate of early gastric cancer. Conclusion SOX4 gene mutation may be related to gastric cancer development and metastasis of relevance. SOX4 of this study was to explore gene mutation and human tumors provided the relationship between the molecular data. [Keywords:] gastric cancer; SOX4; HMG-box; mutation The mutation and sequence analysis of SOX4 in gastric carcinoma [Abstract] Objective To investigate the mutation of SOX4 HMG-box in gastric carcinoma tissues and to explore its roles in progression of gastric carcinoma.Methods The DNA of gastric carcinoma tissues and gastric mucosa tissues were extracted. HMG-BOX of SOX4 gene was amplified by PCR. After PCR-SSCP analyzing, the different samples were sequenced, and translated into peptide, then analyzed by ClustalX software.Results 12 out of 27 gastric carcinoma samples have mutations, no mutations have been detected in the paracancerous tissues, mutation rate is higher in terminal stomach cancer than in early stomach cancer.Conclusion This suggests SOX4 gene mutations have some relation with the happening o