Gentiopicroside mouse tissue content of liquid chromatography - tandem mass spectrometry.docVIP

 PAGE \* MERGEFORMAT 12 Gentiopicroside mouse tissue content of liquid chromatography - tandem mass spectrometry [Abstract] Objective To develop a liquid chromatography - tandem mass spectrometry (LC-MS/MS) determination of liver, heart, spleen, lung and kidney tissue concentrations of gentiopicroside method basis for tissue distribution studies. Methods Samples treatment using solid phase extraction, internal standard, paracetamol, Column: Kromasil C8 column (3.5μm, 2.1 mm × 150 mm), mobile phase was methanol -1% formic acid - acetonitrile (60:30:10), flow rate 170 μl · min-1, ionized by ESI and positive ion multiple reaction monitoring mode detection. The results of the determination of gentiopicroside linear range in the organization 3 ~ 3 000 ng · ml-1, detection limit 3 ng · ml- 1, the chromatographic peak retention time of 2.40 min, recoveries of the organizations were 89.2% ~ 107.2%, intra and inter precision RSD value of less than 12% extraction recovery was more than 78%. Conclusion The method is accurate, convenient, fast, high sensitivity, high-volume tissue samples for gentiopicroside content. [Keywords:] gentiopicroside; liquid chromatography - tandem mass spectrometry; organization; determination Abstract: ObjectiveTo establish LC-MS/MS determination method of gentiopicroside in liver, heart, spleen, lung and kidney in mice. MethodsTissue samples were prepared based on solid-phase extraction with Paracetamol as internal standard, and separation was made through a Kromasil C8 column (3.5μm, 2.1 mm × 150mm), the mobile phase consisted of methanol-1% Formic acid-acetonitrile (60:30:10) with the flow rate of 0.17 ml · min-1. The sample was detected by the positive electrospray ionization (ESI)-MS method under multiple reaction monitor (MRM) mode.ResultsThe linearity of gentiopicroside ranged from 3 to 3000 ng · ml-1, the detection limit was 3 ng · ml-1. The recovery of this method ranged from 89.2% ~ 107.2%. The value of RSD of intraday and i