Genuine medicine Dipsacus genomic DNA extraction methods to improve.docVIP

 PAGE \* MERGEFORMAT 13 Genuine medicine Dipsacus genomic DNA extraction methods to improve [Abstract] Objective To study the genuine medicine Dipsacus genetic diversity, species identification and gene fingerprinting to provide the basic guarantee for the construction of such. Approach to medicine Chinese herbal medicine Dipsacus source fresh leaves of plants, the use of CTAB and improved method to extract genomic DNA from (DNA), and to detect its extraction. the results using the modified CTAB method to extract from fresh leaves of Dipsacus higher concentration of genomic DNA, PCR reactions can meet the requirements. Conclusion The modified CTAB self-extracting genuine medicine Dipsacus better genomic DNA. [Keywords:] Dipsacus; deoxyribonucleic acid (DNA) extraction; CTAB method; Improvement Genuine culture of traditional Chinese medicine herbs in the boutique, is the external environment in specific areas and production areas where the species is a combination of factors such as genetic traits under the influence of the formation of good quality, good curative effect of Chinese herbal medicines [1 ]. authentic medicinal herbs and non-authentic producing areas of raw materials are different, differences in quality of herbs, but the two differences in the morphology and tissue structure is often not obvious, difficult to use traditional methods of accurate identification of authentic ingredients. With the molecular biology the development of science, DNA molecular identification techniques and methods have been widely used in Chinese herbal medicines and non-authentic nature of the genuineness of the identification [2]. and Purification of DNA is the DNA and other molecular biology molecular identification of the important steps is also Molecular diagnostic techniques of medicinal plants in the key. Because herbal medicines contain a small molecule secondary metabolites, such as terpenes, flavonoids, coumarins, organic acids, tannins and other compoun