Hawthorn sperm quality standard lipid-lowering Capsules.docVIP

 PAGE \* MERGEFORMAT 12 Hawthorn sperm quality standard lipid-lowering Capsules Author: ZHU Cai-qing Li Yan Di Nan-Yi Yu Hua Zeng Health [Abstract] Objective To hawthorn herbs and ursolic acid as the control reference substance, respectively, the establishment of thin-layer chromatography (TLC) to identify and high-performance liquid chromatography (HPLC) method Control of lipid-lowering Capsules Hawthorn fine quality. TLC identification method using toluene - ethyl acetate - formic acid (20:4:0.5) as mobile phase; HPLC method using Alltima C18 column (5 μ m, 4.6 mm * 250 mn), with acetonitrile - methanol - water - ammonium acetate ( 70:16:14: 0.5) as mobile phase, detection wavelength 220 nm; column temperature was 25 ℃ ; flow rate of 1.0 ml / min. The results of TLC spots were clear identification of patterns, and negative non-interference; ursolic acid content of 1.796 8 ~ 17.968 0 μ g was well within the linear relationship between the regression equation was Y = 44 820X 5 725.5, r = 0.999 9, average recovery and RSD were 101.17% and 1.02%. Conclusion ursolic acid as a reference substance, using TLC method and HPLC method Hawthorn precise control of the quality of lipid-lowering capsule method is simple, rapid, accurate and reliable, reproducible, specific. [Keywords:] Hawthorn fine Jiangzhi Capsule TLC ursolic acid by high performance liquid chromatography Hawthorn fine Jiangzhi Capsule is made of hawthorn extract unilateral preparation of Chinese medicine is determined by Hawthorn fine piece by the formulations change from lipid-lowering, function and indications for lowering blood pressure, can be used to treat high blood lipid disorders, may be used as coronary heart disease and the adjuvant treatment of hypertension [1]. Reported in the literature mainly hawthorn flavonoids containing organic acids and triterpenoids and other active ingredients, including hyperin, ursolic acid is a hawthorn flavonoids and triterpenes in the main component of the ma