High glucose on human peritoneal mesothelial cells gap junction protein.docVIP

 PAGE \* MERGEFORMAT 12 High glucose on human peritoneal mesothelial cells gap junction protein Of: Caodong Wei, Zhang Miao, He Jinsong, Jiang Chunming [Abstract] Objective To study the high glucose on human peritoneal mesothelial cell gap junction protein (connexin43) in rats. Isolated cultured human peritoneal mesothelial cells, cells were divided into 3 groups: normal glucose group (containing 5.5 mmol / L glucose), high glucose group (containing 140 mmol / L glucose) and osmotic pressure of the control group (containing 5.5 mmol / L glucose plus 24.5 mmol / L mannitol), 24,48 h culture, the application of Northern blot, Western blot to detect connexin43 mRNA and protein expression, the difference between the 3 groups. Results normal glucose peritoneal mesothelial cells rich in connexin43, high glucose in cultured peritoneal mesothelial cells express connexin43 mRNA and protein were significantly higher than normal glucose decreased (P lt;0.05 .) osmotic pressure and the normal control group, no significant difference between the glucose group (Pgt; 0.05). Conclusion High glucose inhibits human peritoneal mesothelial cells connexin43 mRNA and protein expression may be as peritoneal dialysis (PD) in the cortical integrity of the peritoneal injury one of the mechanisms. [Keywords:] peritoneal mesothelial cells; connexin43; peritoneal dialysis Abstract: Objective To investigate the effect of the high glucose concentration on the connexin43 expression in the cultured human peritoneal mesothelial cells (HPMC). Methods HPMCs isolated and cultured in vitro were divided into normal glucose group (5.5 mmol / L), high glucose group (140 mmol / L) and osmotic control group (5.5 mmol / L glucose group plus 24.5 mmol / L mannitol). At 24 h and 48 h of culture, the expression of connexin43 mRNA and protein was determined by Northern blot and Western blot, and the differences between the 3 groups were compared. Results HPMC in the normal glucose group expressed