High Performance Liquid Chromatography less hair in the North Peucedanum coumarin 6.docVIP

 PAGE \* MERGEFORMAT 13 High Performance Liquid Chromatography less hair in the North Peucedanum coumarin 6 Of: Chen Erlin Liu Song Pingshun flowers Hufang Di Li Feng Swaziland [Abstract] less hair for the simultaneous determination of of 6 North Peucedanum coumarin (Hong orange lactone, onion skin pigment, silk Li Nepal Pavilion, Shun 3 ‘, 4’ two Senecio acyl 3 ‘, 4’-dihydro- Artemisia evil lactone, D Pd and Pd prime factor E) content of the HPLC analysis. The column was Lot Validation C18 (150 mm × 4.6 mm, 5 μm) column; mobile phase was methanol water; flow rate of 0.8 mL / min; detection wavelength was 323 nm; column temperature was 30 ℃ .6 coumarins good linearity in a certain range, the correlation coefficient was 0.9992 ~ 0.9999; precision RSD were lower than 3.95%; the average recovery was 93.0% 98.6%. The method is simple, rapid and accurate; and one of the coumarin compounds were isolated by NMR and MS spectra used to confirm the structure of this compound. [Keywords:] less hair North Peucedanum, coumarin, high performance liquid chromatography, the structure 1 Introduction Peucedanum (Radix Peucedani) is a widely used traditional Chinese medicine, genuine Umbelliferae Peucedanum Peucedanum as an Pecucedanum Pd (P.praerutorum) of the dried root of Peucedanum of modern pharmacological studies have shown bronchitis, high blood pressure , coronary heart disease have a certain effect [1]. less hair North Peucedanum (Peucedunum harry smithii var subglabrum), Xi said rod Peucedanum goods, mainly produced in Gansu Province, Longnan and Tianshui, Gansu and other regions [2] . Coumarins are the main active ingredient imperatorin has a genuine determination of total coumarins in Peucedanum and some coumarin content of [3 5], and for less hair in the North Peucedanum not the determination of coumarin reported. The establishment of the HPLC determination of less hair in the North Peucedanum coumarin 6 (Hong orange lactone, onion skin pigment, sil