High Performance Liquid Chromatography Gengnianle Icariin Capsules.docVIP

 PAGE \* MERGEFORMAT 12 High Performance Liquid Chromatography Gengnianle Icariin Capsules 【Abstract】 Objective To establish Gengnianle capsule method for determination of icariin. Methods using high performance liquid chromatography with C18 column (5 μm, 4.6mm × 200 mm); acetonitrile: water (30:70) as mobile phase; flow rate of 1.0 ml / min, detection wavelength 270 nm. The results of icariin in the range of 0.204 ~ 0.816 μg showed a good linear relationship within, r = 0.999 9. The average recovery was 98.77%, RSD 1.58%. Conclusion The method is simple, sensitive and can be used Icariin gengnianle capsules by HPLC. Keywords: gengnianle capsules; icariin; high-performance liquid chromatography Abstract: ObjectiveTo establish HPLC method for the determination of Icariine in Gengnianle Capsule. Methods LUNA ODS C18 BDS (200 mm × 4.6 mm) was used with acetonitrile water (30:70) as a mobile phase. The flow rate was 1.0 ml * min -1. The detecting wavelength was 270 nm. ResultsThe linear range of Icariine was 0.201 ~ 0.816 μg, r = 0.999 9. The average recovery was 98.77%. Conclusion The method is simple, rapid and accurate. Keywords:: Gengnianle Capsule; Icariine; HPLC Gengnianle capsules from epimedium, oysters, Anemarrhena, Rosa laevigata, cork, plantain seed, ginseng etc., with Yang Xin Yang Shen, regulate and fortify the Chong and Ren function. Menopause occurs for the night sleep soundly anxiety, palpitations, tinnitus, suspicious sentimental, Hongre sweating, irritability irritability, back pain embolism. According to Fang composition, to choose their primary drug of Epimedium icariin content in the control of a target, using high performance liquid chromatography preparation of icariin. Are reported below. An instrument and reagent HP1100 high performance liquid chromatography, VWD UV detector, HP ChemStation. Column: LUNA ODS C18 column (200 mm × 4.6 mm, 5 μm); ultrasonic extractor (AS3120B).