High Performance Liquid Chromatography Niuhuang Qingwei pill contents of chlorogenic acid.docVIP

 PAGE \* MERGEFORMAT 12 High Performance Liquid Chromatography Niuhuang Qingwei pill contents of chlorogenic acid 【Abstract】 Objective: To establish a bezoar Qingwei pill method for the determination. Methods: The high-performance liquid chromatography. Column: Agilent Technologies ZORBAX Extend-C18 4.6 × 250mm, 5μm, mobile phase: methanol-0.1mol / L sodium dihydrogen phosphate buffer (pH = 2.7) (70:30, V / V), flow rate: 1.0 ml * min-1, detection wavelength: 328nm, column temperature: room temperature (22 ℃). Results: chlorogenic acid in the 0.01 ~ 0.25mg * ml-1 range of a good linear relationship (r = 0.9998); The average recovery was 98.3%, RSD = 1.4% (n = 6). Conclusion: This method is simple, accurate and reliable, suitable for quality control pills Niuhuang Qingwei. Keywords: high-performance liquid chromatography bezoar Qingwei pill Determination of chlorogenic acid Determination of Chlorogenic Acid in Niuhuangqinwei pills by HPLC 【Abstract objective】 To set up a method for quality control of Niuhuangqingwei pills. METHOD HPLC method was developed to quantitative determination. Agilent Technologies ZORBAX Extend-C18 4.6 × 250mm, 5μm). The mobilic phase was methanol-0.1mol phosphoric [NaH2PO4] (70:30 V / V). The column temperature was 22 ℃, the wavelength for detection was 328nm, flow rate was 1.0ml * min-1. RESULTS The Chlorogenic Acid; average of recovery rate was 98.3%, and RSD was 1.4% (n = 6). CONCLUSION The method is simple, accurate and suitable for its assaying. 【Keywords:】 HPLC; Niuhuangqingwei pills; Chlorogenic Acid; determination Niuhuang Qingwei pill as “the Ministry of Health Drug Standards,” Volume I (TCD Narikata preparation) containing relevant varieties, from the bezoar, chrysanthemum, Scutellaria, Phellodendron, Campanulaceae, Ophiopogon japonicus and other 17 Chinese herbal medicines, has a Qingwei diarrhea fire, the effectiveness of Runzao laxative. For cardiac stomach fire clinical Sheng,