High Performance Liquid Chromatography of herpes Kang sprays of berberine hydrochloride in.docVIP

 PAGE \* MERGEFORMAT 9 High Performance Liquid Chromatography of herpes Kang sprays of berberine hydrochloride in [Abstract] Objective To develop a herpes health of berberine hydrochloride spray method for the determination. Methods using high performance liquid chromatography, Betasil C18 column to column, mobile phase of acetonitrile-glacial acetic acid    water-triethylamine (volume ratio 28:70:1:1), the detection wavelength of 265 nm, column temperature: 30 ℃ . Results berberine 0.1026 ~ 1.0260 μ g showed good linear relationship within the framework of, r = 0.9998, the lowest limit of quantification of 0.1026 μ g, the average recovery was 103.81%, RSD 0.78%, intermediate precision RSD = 0.97% . Conclusion The method can accurately and quickly determine herpes Kang content of berberine hydrochloride spray can be used for the preparation of quality control. [Keywords:] Herpes Berberine Hydrochloride Kang spray high-performance liquid chromatography assay Abstract: Objective To establish a method for determining the content of berberine hydrochloride in Paozhenkang spray. Methods Berberine hydrochloride in the spray was analyzed by HPLC (Betasil C18 column, acetonitrile  water  glacial acetic acid  triethylamine (28:70:1:1 ) as mobile phase, detection wavelength 265 nm, column temperature 30 ℃ ). Results The linear range of berberine hydrochloride was 0.1026 ~ 1.0260 μ g (r = 0.9998) and the detection limit 0.1026 μ g with the average recovery 103.81% (RSD 0.78%) . Conclusion An analytical method is established to measure the concentration of berberine hydrochloride, which could be used for the quality control of Paozhenkang spray. Keywords:: Pozhenkang spray; berberine hydrochloride; HPLC Herpes Kang spray from berberine, rhubarb, Cimicifuga, mountain Arrowhead drugs such as composition, is the clinical experience side. With anti-zoster virus role in promoting the shingles, bed sores and eczema rehabilitation, to alleviate the resulting neur