High Performance Liquid Chromatography Shenqi Capsule gastrodin content.docVIP

 PAGE \* MERGEFORMAT 6 High Performance Liquid Chromatography Shenqi Capsule gastrodin content [Abstract] Objective: To determine the effective ingredients Shenqi capsule method. Methods: HPLC for the content of Gastrodia elata Shenqi capsule were determined. Results: gastrodin the 0.0832 μ g-0.037512 μ g good linear relationship between the average recovery was 100.5%, RSD = 1.65% (n = 5). Conclusion: This method is sensitive, good reproducibility and is suitable for Shenqi capsule assay of Gastrodia elata. [Keywords:] gastrodin / separation and extraction; chromatography, high pressure liquid; ginseng; Astragalus Shenqi capsule from ginseng, astragalus, Tianma composed of 11 Herbs, with qi nourishing the blood, spleen kidney efficacy, Tianma, as one of the main drug, its active ingredients as the gastrodin, as the preparation contains more than Medicine Smell, composition complex and interfere with each other big, so through the test, set up high-performance liquid chromatography Shenqi Capsule gastrodin content by methodological study confirmed the high specificity of the method, the recovery and good precision. An experimental material LC  2010 high-performance liquid chromatograph, SPD  M10Avp diode array detector, LC  10ATvp high-performance liquid chromatograph, SPD  10Avp UV detector, CLASS  VP workstation, N  2000 chromatography workstation. Gastrodin reference standards were purchased from China Pharmaceutical and Biological Products Institution, pure methanol for chromatography and other reagents were analytical pure. 2 Experimental Methods and Results 2.1 Chromatographic conditions [1  2] Chromatography: Aglient C18 (4.6 mm * 250 mm, 5 μ m); mobile phase: methanol  0.1% phosphoric acid (five ninety-five); flow rate: 1.0 mL / min; column temperature 25 ℃ ; detection wavelength: 220 nm; injection volume 20 μ L. 2.2 Preparation of reference substance solutions that take gastrodin precise amount of reference substance, add ace