High Performance Liquid Chromatography Shenqi Yinao capsule of β -asarone content of.docVIP

 PAGE \* MERGEFORMAT 10 High Performance Liquid Chromatography Shenqi Yinao capsule of β -asarone content of [Abstract] Objective To establish the parameters Qi Yi Nao Capsule β -asarone in high-performance liquid chromatography assay method. Method column was Thermo BDS Hypersil C18 (250 mm * 4.6 mm, 5 μ m); mobile phase was methanol -0.1% acetic acid solution (60:40); column temperature 35 ℃ ; flow rate of 1.0 ml * min-1; detection wavelength to 257 nm. Results β -asarone concentration of 4.885 ~ 146.55 μ g / ml between the peak area and concentration has a good linear relationship. Its high, medium and low concentrations of three kinds of average recoveries were 103.2%, 102.1% and 100.2%; RSD were 0.4%, 0.3% and 0.7%. CONCLUSION This method is simple and sensitive, accurate and reliable, specific and can be used to Shenqi Yinao Capsule Sweetflag quality control. [Keywords:] parameter Qi Yi Nao Capsule β -asarone in Acorus gramineus high performance liquid chromatography Abstract: ObjectiveTo establish a high performance liquid chromatographic method for the determination of β -asarone in Shenqiyinao Capsules. MethodsThe column was Thermo BDS Hypersil C18 (250 mm * 4.6 mm, 5 μ m). The mobile phase was methanol - 0.1% glacial acetic acid solution (60:40) and the temperature of column was 35 ℃ . The flow rate was 1.0 ml * min-1 and the detection wavelength was 257 nm with external method. ResultsThe linear range of β -asarone was 4.885 ~ 146.55 μ g * ml -1. The average recoveries of β -asarone were 103.2%, 102.1% and 100.2% with corresponding RSD of 0.4%, 0.3% and 0.7%, respectivery. ConclusionThe method is simple, sensitive, accurate and can be used for the determination of Rhizoma Acori Tatarinowii (RAT) in Shenqiyinao Capsules. Keywords:: Shenqiyinao Capsules; β -asarone; Rhizoma Acori Tatarinowii (RAT); HPLC Shenqi Yinao capsule replenishing liver and kidney, Qi and blood, brain puzzle features such as lack of suitable liver and kidney, brain God